Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR.

ABSTRACT:

Background

Circulating tumor cells (CTCs) have been associated with prognosis especially in breast cancer and have been proposed as a liquid biopsy for repeated follow up examinations. Molecular characterization of CTCs is difficult to address since they are very rare and the amount of available sample is very limited.

Methods

We quantified by RT-qPCR CK-19, MAGE-A3, HER-2, TWIST1, hTERT α+β+, and mammaglobin gene transcripts in immunomagnetically positively selected CTCs from 92 breast cancer patients, and 28 healthy individuals. We also compared our results with the CellSearch system in 33 of these patients with early breast cancer.

Results

RT-qPCR is highly sensitive and specific and can detect the expression of each individual gene at the one cell level. None of t

SUBMITTER: Strati A 

PROVIDER: S-EPMC3224356 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature