BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).

ABSTRACT:

Background

Although second generation sequencing (2GS) technologies allow re-sequencing of previously gold-standard-sequenced genomes, whole genome shotgun sequencing and de novo assembly of large and complex eukaryotic genomes is still difficult. Availability of a genome-wide physical map is therefore still a prerequisite for whole genome sequencing for genomes like barley. To start such an endeavor, large insert genomic libraries, i.e. Bacterial Artificial Chromosome (BAC) libraries, which are unbiased and representing deep haploid genome coverage, need to be ready in place.

Result

Five new BAC libraries were constructed for barley (Hordeum vulgare L.) cultivar Morex. These libraries were constructed in different cloning sites (HindIII, EcoRI, MboI and BstXI) of the respec

SUBMITTER: Schulte D 

PROVIDER: S-EPMC3224359 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature