Approximate and exact equations for peak capacity in isocratic high-pressure liquid chromatography.

ABSTRACT: In this letter, we examine an equation that had been published in Analytical Chemistry in a paper entitled "Comprehensive Study on the Optimization of Online Two-Dimensional Liquid Chromatographic Systems Considering Losses in Theoretical Peak Capacity in First- and Second-Dimensions: A Pareto-Optimality Approach" by Vivo-Truyols, G.; van der Wal, Sj.; Schoenmakers, P. J. Anal. Chem. 2010, 82, 8525-8536. In that paper, the authors considered, among many issues, the effects of extra-column and column broadening on isocratic peak capacity. They developed an equation to cover all possible conditions and offered a derivation based on two Taylor-series expansions and a regression. We have found an exact equation that covers all conditions and have compared the results using our equation to the

SUBMITTER: Potts LW 

PROVIDER: S-EPMC3224533 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature