Reference gene validation for qPCR in rat carotid body during postnatal development.

ABSTRACT:

Background

The carotid bodies are the main arterial oxygen chemoreceptors in mammals. Afferent neural output from the carotid bodies to brainstem respiratory and cardiovascular nuclei provides tonic input and mediates important protective responses to acute and chronic hypoxia. It is widely accepted that the selection of reference genes for mRNA normalization in quantitative real-time PCR must be validated for a given tissue and set of conditions. This is particularly important for studies in carotid body during early postnatal maturation as the arterial oxygen tension undergoes major changes from fetal to postnatal life, which may affect reference gene expression. In order to determine the most stable and suitable reference genes for the study of rat carotid body during developmen

SUBMITTER: Kim I 

PROVIDER: S-EPMC3224571 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature