Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line.

ABSTRACT:

Background

Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2) as compared to normal African green monkey kidney epithelial cell lines.

Methods

Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the HepG2 cells with diamidino-2-phenylindole staining. DNA fragmentation, indicative of apoptosis, was analyzed with agarose gel electrophoresis. Alkylation, indicative of DNA damage, was also evaluated in vitro by 4-(4'-nitrobenzyl) pyridine assay.

Results

The extract of Pinus kesiya showed the highest selectivity (selectivity index = 9.6) and potent cytotoxicity in the HepG2 cell

SUBMITTER: Machana S 

PROVIDER: S-EPMC3224580 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature