Plasma proteome changes in cardiovascular disease patients: novel isoforms of apolipoprotein A1.

ABSTRACT:

Background

The aim of this proteomic study was to look for changes taking place in plasma proteomes of patients with acute myocardial infarction (AMI), unstable angina pectoris (UAP), and stable angina pectoris (SAP).

Methods

Depleted plasma proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Proteins were quantified using commercial kits. Apolipoprotein A1 was studied using 1D and 2D SDS-PAGE, together with western blotting.

Results

Reciprocal comparison revealed 46 unique, significantly different spots; proteins in 34 spots were successfully identified and corresponded to 38 different proteins. Discrete comparisons of patient groups showed 45, 41, and

SUBMITTER: Majek P 

PROVIDER: S-EPMC3224581 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature