Selection of reliable reference genes for gene expression studies in peach using real-time PCR.

ABSTRACT:

Background

RT-qPCR is a preferred method for rapid and reliable quantification of gene expression studies. Appropriate application of RT-qPCR in such studies requires the use of reference gene(s) as an internal control to normalize mRNA levels between different samples for an exact comparison of gene expression level. However, recent studies have shown that no single reference gene is universal for all experiments. Thus, the identification of high quality reference gene(s) is of paramount importance for the interpretation of data generated by RT-qPCR. Only a few studies on reference genes have been done in plants and none in peach (Prunus persica L. Batsch). Therefore, the present study was conducted to identify suitable reference gene(s) for normalization of gene expression in pea

SUBMITTER: Tong Z 

PROVIDER: S-EPMC3224724 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature