Identification of target genes of transcription factor activator protein 2 gamma in breast cancer cells.

ABSTRACT:

Background

Activator protein 2 gamma (AP-2gamma) is a member of the transcription factor activator protein-2 (AP-2) family, which is developmentally regulated and plays a role in human neoplasia. AP-2gamma has been found to be overexpressed in most breast cancers, and have a dual role to inhibit tumor initiation and promote tumor progression afterwards during mammary tumorigensis.

Methods

To identify the gene targets that mediate its effects, we performed chromatin immunoprecipitation (ChIP) to isolate AP-2gamma binding sites on genomic DNA from human breast cancer cell line MDA-MB-453.

Results

20 novel DNA fragments proximal to potential AP-2gamma targets were obtained. They are categorized into functional groups of carcinogenesis, metabolism and others. A combinatio

SUBMITTER: Ailan H 

PROVIDER: S-EPMC3224728 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature