Effects of cyclooxygenase-2 on human esophageal squamous cell carcinoma.

ABSTRACT:

Aim

To study the relationship between the cyclooxygenase (COX)-2 gene and the proliferation and apoptosis of esophageal squamous carcinoma EC109 cells.

Methods

The techniques of RNA interference (RNAi) and cell transfection, as well as the levels of oncogenicity in nude mice, were used to study the role of COX-2 in the esophageal squamous carcinoma cell (ESCC) line EC109. Following RNAi and transfection, Western blotting analysis was used to determine the expression of the COX-2 protein. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) reduction assay was used to evaluate cell growth, and flow cytometry was used to detect cell apoptosis.

Results

Western blotting analysis demonstrated that COX-2 expression was significantly reduced in EC109 cells

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3225093 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature