A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors.

ABSTRACT:

Background

Fluorescent protein (FP)-based biosensors based on the principle of intramolecular Förster resonance energy transfer (FRET) enable the visualization of a variety of biochemical events in living cells. The construction of these biosensors requires the genetic insertion of a judiciously chosen molecular recognition element between two distinct hues of FP. When the molecular recognition element interacts with the analyte of interest and undergoes a conformational change, the ratiometric emission of the construct is altered due to a change in the FRET efficiency. The sensitivity of such biosensors is proportional to the change in ratiometric emission, and so there is a pressing need for methods to maximize the ratiometric change of existing biosensor constructs in order to i

SUBMITTER: Ibraheem A 

PROVIDER: S-EPMC3225322 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature