Quantitative proteomics reveals metabolic and pathogenic properties of Chlamydia trachomatis developmental forms.

ABSTRACT: Chlamydia trachomatis is an obligate intracellular pathogen responsible for ocular and genital infections of significant public health importance. C. trachomatis undergoes a biphasic developmental cycle alternating between two distinct forms: the infectious elementary body (EB), and the replicative but non-infectious reticulate body (RB). The molecular basis for these developmental transitions and the metabolic properties of the EB and RB forms are poorly understood as these bacteria have traditionally been difficult to manipulate through classical genetic approaches. Using two-dimensional liquid chromatography - tandem mass spectrometry (LC/LC-MS/MS) we performed a large-scale, label-free quantitative proteomic analysis of C. trachomatis LGV-L2 EB and RB forms. Additionally, we carried ou

SUBMITTER: Saka HA 

PROVIDER: S-EPMC3225693 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature