Spotted cotton oligonucleotide microarrays for gene expression analysis.

ABSTRACT:

Background

Microarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species. Using these assemblies as templates, we describe the design and creation and of a publicly available oligonucleotide array for cotton, useful for all four of the cultivated species.

Results

Synthetic oligonucleotide probes were generated from exemplar sequences of a global assembly of 211,397 cotton ESTs derived from >50 different cDNA libraries representing many different tissue types and tissue treatments. A total of 22,787 oligonucleotide probes are included on the arrays, optimized to target the diversity of the transcriptome and previously studied cotton genes, t

SUBMITTER: Udall JA 

PROVIDER: S-EPMC3225879 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature