Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.

ABSTRACT:

Introduction

The controlled expression of many genes, including G-protein coupled receptors (GPCRs), is important for delineating gene functions in complex model systems. Binary systems for inducible regulation of transgene expression are widely used in mice. One system is the tTA/TRE expression system, composed of a tetracycline-dependent DNA binding factor and a separate tetracycline operon. However, the requirement for two separate transgenes (one for each tTA or TRE component) makes this system less amenable to models requiring directed cell targeting, increases the risk of multiple transgene integration sites, and requires extensive screening for appropriately-functioning clones.

Methods

We developed a single, polycistronic tetracycline-inducible expression platform to

SUBMITTER: Hsiao EC 

PROVIDER: S-EPMC3226282 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature