A genome-wide screen for Neurospora crassa transcription factors regulating glycogen metabolism.

ABSTRACT: Transcription factors play a key role in transcription regulation as they recognize and directly bind to defined sites in promoter regions of target genes, and thus modulate differential expression. The overall process is extremely dynamic, as they have to move through the nucleus and transiently bind to chromatin in order to regulate gene transcription. To identify transcription factors that affect glycogen accumulation in Neurospora crassa, we performed a systematic screen of a deletion strains set generated by the Neurospora Knockout Project and available at the Fungal Genetics Stock Center. In a wild-type strain of N. crassa, glycogen content reaches a maximal level at the end of the exponential growth phase, but upon heat stress the glycogen content rapidly drops. The gene encoding gl

SUBMITTER: Goncalves RD 

PROVIDER: S-EPMC3226398 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature