A novel esterase gene cloned from a metagenomic library from neritic sediments of the South China Sea.

ABSTRACT:

Background

Marine microbes are a large and diverse group, which are exposed to a wide variety of pressure, temperature, salinity, nutrient availability and other environmental conditions. They provide a huge potential source of novel enzymes with unique properties that may be useful in industry and biotechnology. To explore the lipolytic genetic resources in the South China Sea, 23 sediment samples were collected in the depth < 100 m marine areas.

Results

A metagenomic library of South China Sea sediments assemblage in plasmid vector containing about 194 Mb of community DNA was prepared. Screening of a part of the unamplified library resulted in isolation of 15 unique lipolytic clones with the ability to hydrolyze tributyrin. A positive recombinant clone (pNLE1), containing

SUBMITTER: Peng Q 

PROVIDER: S-EPMC3226443 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature