Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells.

ABSTRACT: Septin family proteins oligomerize through guanosine 5'-triphosphate-binding domains into core heteromers, which in turn polymerize at the cleavage furrow of dividing fungal and animal cells. Septin assemblies during the interphase of animal cells remain poorly defined and are the topic of this report. In this study, we developed protocols for visualization of authentic higher-order assemblies using tagged septins to effectively replace the endogenous gene product within septin core heteromers in human cells. Our analysis revealed that septins assemble into microtubule-supported, disk-like structures at the plasma membrane. In the absence of cell substrate adhesion, this is the predominant higher-order arrangement in interphase cells and each of the seven to eight septin family members exp

SUBMITTER: Sellin ME 

PROVIDER: S-EPMC3226477 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature