Transcriptome analysis of orange-spotted grouper (Epinephelus coioides) spleen in response to Singapore grouper iridovirus.

ABSTRACT:

Background

Orange-spotted grouper (Epinephelus coioides) is an economically important marine fish cultured in China and Southeast Asian countries. The emergence of infectious viral diseases, including iridovirus and betanodavirus, have severely affected food products based on this species, causing heavy economic losses. Limited available information on the genomics of E. coioides has hampered the understanding of the molecular mechanisms that underlie host-virus interactions. In this study, we used a 454 pyrosequencing method to investigate differentially-expressed genes in the spleen of the E. coioides infected with Singapore grouper iridovirus (SGIV).

Results

Using 454 pyrosequencing, we obtained abundant high-quality ESTs from two spleen-complementary DNA libraries which

SUBMITTER: Huang Y 

PROVIDER: S-EPMC3226587 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature