Cell-based sensors for quantifying the physiological impact of microsystems.

ABSTRACT: Microsystems are increasingly used in the manipulation, patterning and sorting of cells. Critical to the widespread adoption of these new technologies is development of an understanding of their impact on cellular physiology. Here we show the integration of a cell-based sensor, a microfabricated electrical screening platform, and quantitative imaging to enable the first large-scale physiological screens of the impact of microsystems on cells. To perform physiological screening, we developed a cell-based sensor that reports on stress-mediated transcription (via Heat Shock Factor 1 induced expression of GFP). This cell-based sensor was quantitatively characterized using automated imaging. The integration of this quantitative physiological sensor with a microfabricated system enabled the exec

SUBMITTER: Desai SP 

PROVIDER: S-EPMC3226760 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature