Nonenzymatic assembly of natural polyubiquitin chains of any linkage composition and isotopic labeling scheme.

ABSTRACT: Polymeric chains made of a small protein ubiquitin act as molecular signals regulating a variety of cellular processes controlling essentially all aspects of eukaryotic biology. Uncovering the mechanisms that allow differently linked polyubiquitin chains to serve as distinct molecular signals requires the ability to make these chains with the native connectivity, defined length, linkage composition, and in sufficient quantities. This, however, has been a major impediment in the ubiquitin field. Here, we present a robust, efficient, and widely accessible method for controlled iterative nonenzymatic assembly of polyubiquitin chains using recombinant ubiquitin monomers as the primary building blocks. This method uses silver-mediated condensation reaction between the C-terminal thioester of on

SUBMITTER: Castaneda C 

PROVIDER: S-EPMC3226840 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature