Early events in xenograft development from the human embryonic stem cell line HS181--resemblance with an initial multiple epiblast formation.

ABSTRACT: Xenografting is widely used for assessing in vivo pluripotency of human stem cell populations. Here, we report on early to late events in the development of mature experimental teratoma from a well-characterized human embryonic stem cell (HESC) line, HS181. The results show an embryonic process, increasingly chaotic. Active proliferation of the stem cell derived cellular progeny was detected already at day 5, and characterized by the appearance of multiple sites of engraftment, with structures of single or pseudostratified columnar epithelium surrounding small cavities. The striking histological resemblance to developing embryonic ectoderm, and the formation of epiblast-like structures was supported by the expression of the markers OCT4, NANOG, SSEA-4 and KLF4, but a lack of REX1. The earl

SUBMITTER: Gertow K 

PROVIDER: S-EPMC3227586 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature