Arginylation and methylation double up to regulate nuclear proteins and nuclear architecture in vivo.

ABSTRACT: Protein arginylation and arginine methylation are two posttranslational modifications of emerging importance that involve Arg residues and their modifications. To test a hypothesis that posttranslationally added arginines can be methylated, we used high-precision mass spectrometry and metabolic labeling to find whether posttranslationally added arginines can serve as methylation sites. We identified a number of proteins in vivo, on which posttranslationally added Arg have undergone mono- and dimethylation. This double modification predominantly affects the chromatin-containing nuclear fraction and likely plays an important regulatory role in chromatin-associated proteins. Moreover, inhibition of arginylation and Arg methylation results in a significant reduction of the nucleus size in cult

SUBMITTER: Saha S 

PROVIDER: S-EPMC3227866 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature