Strong cation exchange chromatography in analysis of posttranslational modifications: innovations and perspectives.

ABSTRACT: Strong cation exchange (SCX) chromatography has been utilized as an excellent separation technique that can be combined with reversed-phase (RP) chromatography, which is frequently used in peptide mass spectrometry. Although SCX is valuable as the second component of such two-dimensional separation methods, its application goes far beyond efficient fractionation of complex peptide mixtures. Here I describe how SCX facilitates mapping of the protein posttranslational modifications (PTMs), specifically phosphorylation and N-terminal acetylation. The SCX chromatography has been mainly used for enrichment of these two PTMs, but it might also be beneficial for high-throughput analysis of other modifications that alter the net charge of a peptide.

SUBMITTER: Edelmann MJ 

PROVIDER: S-EPMC3228578 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature