Obscurin depletion impairs organization of skeletal muscle in developing zebrafish embryos.

ABSTRACT: During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the differentiating myocytes and the surrounding extracellular matrix to form correctly positioned and well-organized attachments from the skeletal muscle to the bony skeleton. Using the developing zebrafish embryo as a model, we examined the relationship between new myofibril assembly and the organization of the membrane domains involved in cell-extracellular matrix interactions. We determined that depletion of obscurin, a giant muscle protein, resulted in irregular cell morphology and disturbed extracellul

SUBMITTER: Raeker MO 

PROVIDER: S-EPMC3228690 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature