High resolution melting analysis for the detection of EMS induced mutations in wheat SBEIIa genes.

ABSTRACT:

Background

Manipulation of the amylose-amylopectin ratio in cereal starch has been identified as a major target for the production of starches with novel functional properties. In wheat, silencing of starch branching enzyme genes by a transgenic approach reportedly caused an increase of amylose content up to 70% of total starch, exhibiting novel and interesting nutritional characteristics. In this work, the functionality of starch branching enzyme IIa (SBEIIa) has been targeted in bread wheat by TILLING. An EMS-mutagenised wheat population has been screened using High Resolution Melting of PCR products to identify functional SNPs in the three homoeologous genes encoding the target enzyme in the hexaploid genome.

Results

This analysis resulted in the identification of 56, 14

SUBMITTER: Botticella E 

PROVIDER: S-EPMC3228712 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature