Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons.

ABSTRACT:

Background

Urine within the urinary tract is commonly regarded as "sterile" in cultivation terms. Here, we present a comprehensive in-depth study of bacterial 16S rDNA sequences associated with urine from healthy females by means of culture-independent high-throughput sequencing techniques.

Results

Sequencing of the V1V2 and V6 regions of the 16S ribosomal RNA gene using the 454 GS FLX system was performed to characterize the possible bacterial composition in 8 culture-negative (<100,000 CFU/ml) healthy female urine specimens. Sequences were compared to 16S rRNA databases and showed significant diversity, with the predominant genera detected being Lactobacillus, Prevotella and Gardnerella. The bacterial profiles in the female urine samples studied were complex; considerable

SUBMITTER: Siddiqui H 

PROVIDER: S-EPMC3228714 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature