Comparing the immunosuppressive potency of naive marrow stromal cells and Notch-transfected marrow stromal cells.

ABSTRACT:

Background

SB623 cells are expanded from marrow stromal cells (MSCs) transfected with a Notch intracellular domain (NICD)-expressing plasmid. In stroke-induced animals, these cells reduce infarct size and promote functional recovery. SB623 cells resemble the parental MSCs with respect to morphology and cell surface markers despite having been in extended culture. MSCs are known to have immunosuppressive properties; whether long-term culture of MSCs impact their immunomodulatory activity has not been addressed.

Methods

To assess the possible senescent properties of SB623 cells, we performed cell cycle related assays and beta-galactosidase staining. To assess the immunomodulatory activity of these expanded NICD-transfected MSCs, we performed co-cultures of SB623 cells or MSCs

SUBMITTER: Dao MA 

PROVIDER: S-EPMC3228829 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature