Embryonic stem cell-based mapping of developmental transcriptional programs.

ABSTRACT: The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

SUBMITTER: Mazzoni EO 

PROVIDER: S-EPMC3228994 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature