Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils.

ABSTRACT:

Background

The choice of reliable reference genes is a prerequisite for valid results when analyzing gene expression with real-time quantitative PCR (qPCR). This method is frequently applied to study gene expression patterns in immune cells, yet a thorough validation of potential reference genes is still lacking for most leukocyte subtypes and most models of their in vitro stimulation. In the current study, we evaluated the expression stability of common reference genes in two widely used cell culture models-anti-CD3/CD28 activated T cells and lipopolysaccharide stimulated neutrophils-as well as in unselected untreated leukocytes.

Results

The mRNA expression of 17 (T cells), 7 (neutrophils) or 8 (unselected leukocytes) potential reference genes was quantified by reverse tran

SUBMITTER: Ledderose C 

PROVIDER: S-EPMC3229292 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature