Desorption electrospray ionization then MALDI mass spectrometry imaging of lipid and protein distributions in single tissue sections.

ABSTRACT: Imaging mass spectrometry (MS) is a powerful technique for mapping the spatial distributions of a wide range of chemical compounds simultaneously from a tissue section. Co-localization of the distribution of individual molecular species, including particular lipids and proteins, and correlation with the morphological features of a single tissue section are highly desirable for comprehensive tissue analysis and disease diagnosis. We now report on the use, in turn, of desorption electrospray ionization (DESI), matrix assisted laser desorption ionization (MALDI), and then optical microscopy to image lipid and protein distributions in a single tissue section. This is possible through the use of histologically compatible DESI solvent systems, which allow for sequential analyses of the same sect

SUBMITTER: Eberlin LS 

PROVIDER: S-EPMC3229813 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature