The fine specificity of mannose-binding and galactose-binding lectins revealed using outlier motif analysis of glycan array data.

ABSTRACT: Glycan-binding proteins are commonly used as analytical reagents to detect the levels of specific glycan structures in biological samples. A detailed knowledge of the specificities of glycan-binding proteins is required for properly interpreting their binding data. A powerful technology for characterizing glycan-binding specificity is the glycan array. However, the interpretation of glycan-array data can be difficult due to the complex fine specificities of certain glycan-binding proteins. We developed a systematic approach, called outlier-motif analysis, for extracting fine-specificity information from glycan-array data, and we applied the method to the study of four commonly used lectins: two mannose binders (concanavalin A and Lens culinaris) and two galactose binders (Bauhinia purpurea

SUBMITTER: Maupin KA 

PROVIDER: S-EPMC3230281 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature