RNA targets of wild-type and mutant FET family proteins.

ABSTRACT: FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.

SUBMITTER: Hoell JI 

PROVIDER: S-EPMC3230689 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature