Ectopic proliferation contributes to retinal dysplasia in the juvenile zebrafish patched2 mutant eye.

ABSTRACT:

Purpose

Patched is a well-studied tumor suppressor and negative regulator of the Hedgehog (Hh) pathway. Earlier work in this laboratory has shown that embryonic zebrafish patched2 (ptc2) mutant retinas possess an expanded ciliary marginal zone (CMZ) and phenotypes similar to those in human patients with basal cell naevus syndrome (BCNS), a congenital disorder linked to mutations in the human PTCH gene. This study extends the analysis of retinal structure and homeostasis in ptc2-/- mutants to juvenile stages, to determine whether Patched 2 function is essential in the postembryonic eye.

Methods

Histologic, immunohistochemical, and molecular analyses were used to characterize retinal defects in the 6-week-old juvenile ptc2-/- retina.

Results

Juvenile ptc2-/- mutants exh

SUBMITTER: Bibliowicz J 

PROVIDER: S-EPMC3231842 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature