Identification of the midgut microbiota of An. stephensi and An. maculipennis for their application as a paratransgenic tool against malaria.

ABSTRACT: The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating the mosquito midgut contents from lab-reared and field-collected Anopheles spp. was used for microbiota isolation. The gram-negative and gram-positive bacterial colonies were identified by Gram staining and specific mediums. Selected colonies were identified by differential biochemical tests and 16S

SUBMITTER: Dinparast Djadid N 

PROVIDER: S-EPMC3232223 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature