Adipose tissue extracellular matrix and vascular abnormalities in obesity and insulin resistance.

ABSTRACT:

Context

Insulin resistance is associated with inflammation, fibrosis, and hypoxia in adipose tissue.

Objective

This study was intended to better characterize the extracellular matrix (ECM) and vascularity of insulin-resistant adipose tissue.

Design

Adipose expression of collagens, elastin, and angiogenic factors was assessed using real-time RT-PCR and immunohistochemistry (IHC) in abdominal sc adipose tissue. Adipocyte-macrophage coculture experiments examined the effects of polarized macrophages on adipose ECM gene expression, and the effects of collagens were measured in an angiogenesis assay.

Participants and setting

A total of 74 nondiabetic subjects participated at a University Clinical Research Center.

Interventions

Interventions included baseline

SUBMITTER: Spencer M 

PROVIDER: S-EPMC3232606 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature