Malate-mediated carbon catabolite repression in Bacillus subtilis involves the HPrK/CcpA pathway.

ABSTRACT: Most organisms can choose their preferred carbon source from a mixture of nutrients. This process is called carbon catabolite repression. The Gram-positive bacterium Bacillus subtilis uses glucose as the preferred source of carbon and energy. Glucose-mediated catabolite repression is caused by binding of the CcpA transcription factor to the promoter regions of catabolic operons. CcpA binds DNA upon interaction with its cofactors HPr(Ser-P) and Crh(Ser-P). The formation of the cofactors is catalyzed by the metabolite-activated HPr kinase/phosphorylase. Recently, it has been shown that malate is a second preferred carbon source for B. subtilis that also causes catabolite repression. In this work, we addressed the mechanism by which malate causes catabolite repression. Genetic analyses reveal

SUBMITTER: Meyer FM 

PROVIDER: S-EPMC3232832 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature