Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
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ABSTRACT:
SUBMITTER: Patel M
PROVIDER: S-EPMC3232961 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Publications
Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
Patel Mayank M Gonzalez Rodrigo R Halford Colin C Lewinski Michael A MA Landaw Elliot M EM Churchill Bernard M BM Haake David A DA
Journal of clinical microbiology 20110921 12
We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay. ...[more]
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