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True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.

ABSTRACT: Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens.

SUBMITTER: Rudkjobing VB 

PROVIDER: S-EPMC3233003 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis.

Rudkjøbing Vibeke B VB   Thomsen Trine R TR   Alhede Morten M   Kragh Kasper N KN   Nielsen Per H PH   Johansen Ulla R UR   Givskov Michael M   Høiby Niels N   Bjarnsholt Thomas T  

Journal of clinical microbiology 20111019 12

Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens. ...[more]

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