Evaluation of internal reference genes for quantitative expression analysis by real-time PCR in ovine whole blood.

ABSTRACT: The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and to reduce possible errors in the quantification of gene expression. The most suitable reference genes in sheep have been identified for a restricted range of tissues, but no specific data on whole blood are available. The aim of this study was to identify a set of reference genes for normalizing qRT-PCR from ovine whole blood. We designed 11 PCR assays for commonly employed reference genes belonging to various functional classes and then determined their expression stability in whole blood samples from control and disease-stressed sheep. SDHA and YWHAZ were considered the most suitable internal controls as they were stably expressed regardless of disease status according to both geNorm an

SUBMITTER: Peletto S 

PROVIDER: S-EPMC3233434 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature