IAssembler: a package for de novo assembly of Roche-454/Sanger transcriptome sequences.

ABSTRACT:

Background

Expressed Sequence Tags (ESTs) have played significant roles in gene discovery and gene functional analysis, especially for non-model organisms. For organisms with no full genome sequences available, ESTs are normally assembled into longer consensus sequences for further downstream analysis. However current de novo EST assembly programs often generate large number of assembly errors that will negatively affect the downstream analysis. In order to generate more accurate consensus sequences from ESTs, tools are needed to reduce or eliminate errors from de novo assemblies.

Results

We present iAssembler, a pipeline that can assemble large-scale ESTs into consensus sequences with significantly higher accuracy than current existing assemblers. iAssembler employs MIRA an

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC3233632 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature