Differential regulation of two closely related integrative and conjugative elements from Streptococcus thermophilus.

ABSTRACT:

Background

Two closely related ICEs, ICESt1 and ICESt3, have been identified in the lactic acid bacterium Streptococcus thermophilus. While their conjugation and recombination modules are almost identical (95% nucleotide identity) and their regulation modules related, previous work has demonstrated that transconjugants carrying ICESt3 were generated at rate exceeding by a 1000 factor that of ICESt1.

Results

The functional regulation of ICESt1 and ICESt3 transcription, excision and replication were investigated under different conditions (exponential growth or stationary phase, DNA damage by exposition to mitomycin C). Analysis revealed an identical transcriptional organization of their recombination and conjugation modules (long unique transcript) whereas the transcriptional

SUBMITTER: Carraro N 

PROVIDER: S-EPMC3234194 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature