Uncultivated microbial eukaryotic diversity: a method to link ssu rRNA gene sequences with morphology.

ABSTRACT: Protists have traditionally been identified by cultivation and classified taxonomically based on their cellular morphologies and behavior. In the past decade, however, many novel protist taxa have been identified using cultivation independent ssu rRNA sequence surveys. New rRNA "phylotypes" from uncultivated eukaryotes have no connection to the wealth of prior morphological descriptions of protists. To link phylogenetically informative sequences with taxonomically informative morphological descriptions, we demonstrate several methods for combining whole cell rRNA-targeted fluorescent in situ hybridization (FISH) with cytoskeletal or organellar immunostaining. Either eukaryote or ciliate-specific ssu rRNA probes were combined with an anti-α-tubulin antibody or phalloidin, a common actin sta

SUBMITTER: Hirst MB 

PROVIDER: S-EPMC3234254 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature