Cell-free protein expression under macromolecular crowding conditions.

ABSTRACT:

Background

Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein synthesis in vitro, but also limit our understanding of the functions and interactions of biomolecules involved in this fundamental biological process.

Methodology/principal findings

Using cell-free synthesis of Renilla luciferase in wheat germ extract as a model system, we investigated the CFPE under macromolecular crowding environments emulated with three different crowding agents: PEG-8000, Ficoll-70 and Ficoll-400, which vary in chemical properties and molecular size. We found th

SUBMITTER: Ge X 

PROVIDER: S-EPMC3234285 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature