PcrEfficiency: a Web tool for PCR amplification efficiency prediction.

ABSTRACT:

Background

Relative calculation of differential gene expression in quantitative PCR reactions requires comparison between amplification experiments that include reference genes and genes under study. Ignoring the differences between their efficiencies may lead to miscalculation of gene expression even with the same starting amount of template. Although there are several tools performing PCR primer design, there is no tool available that predicts PCR efficiency for a given amplicon and primer pair.

Results

We have used a statistical approach based on 90 primer pair combinations amplifying templates from bacteria, yeast, plants and humans, ranging in size between 74 and 907 bp to identify the parameters that affect PCR efficiency. We developed a generalized additive model fitt

SUBMITTER: Mallona I 

PROVIDER: S-EPMC3234296 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature