Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters.

ABSTRACT:

Unlabelled

The human cytomegalovirus and elongation factor 1α promoters are constitutive promoters commonly employed by mammalian expression vectors. These promoters generally produce high levels of expression in many types of cells and tissues. To generate a library of synthetic promoters capable of generating a range of low, intermediate, and high expression levels, the TATA and CAAT box elements of these promoters were mutated. Other promoter variants were also generated by random mutagenesis. Evaluation using plasmid vectors integrated at a single site in the genome revealed that these various synthetic promoters were capable of expression levels spanning a 40-fold range. Retroviral vectors were equipped with the synthetic promoters and evaluated for their ability to reproduce

SUBMITTER: Ferreira JP 

PROVIDER: S-EPMC3234317 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature