Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes.

ABSTRACT: MraY translocase catalyzes the first committed membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I. The essential membrane protein therefore has a high potential as target for drug screening approaches to develop antibiotics against gram-positive as well as gram-negative bacteria. However, the production of large integral membrane proteins in conventional cellular expression systems is still very challenging. Cell-free expression technologies have been optimized in recent times for the production of membrane proteins in the presence of detergents (D-CF), lipids (L-CF), or as precipitates (P-CF). We report the development of preparative scale production protocols for the MraY homologues of Escherichia coli and Bacillus subtilis in all three cell-free

SUBMITTER: Ma Y 

PROVIDER: S-EPMC3234709 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature