Construction and evaluation of a whole genome microarray of Chlamydomonas reinhardtii.

ABSTRACT:

Background

Chlamydomonas reinhardtii is widely accepted as a model organism regarding photosynthesis, circadian rhythm, cell mobility, phototaxis, and biotechnology. The complete annotation of the genome allows transcriptomic studies, however a new microarray platform was needed. Based on the completed annotation of Chlamydomonas reinhardtii a new microarray on an Agilent platform was designed using an extended JGI 3.1 genome data set which included 15000 transcript models.

Results

In total 44000 probes were determined (3 independent probes per transcript model) covering 93% of the transcriptome. Alignment studies with the recently published AUGUSTUS 10.2 annotation confirmed 11000 transcript models resulting in a very good coverage of 70% of the transcriptome (17000). Follo

SUBMITTER: Toepel J 

PROVIDER: S-EPMC3235179 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature