Human keratinocytes' response to injury upregulates CCL20 and other genes linking innate and adaptive immunity.

ABSTRACT: In the early stages of wound healing, keratinocytes (KCs) become "activated" and release inflammatory molecules such as IL-1 and IL-8, which are linked to innate immune responses and neutrophil recruitment. It is unclear, however, whether KCs release molecules linked to adaptive immune responses, e.g., CCL20, in their early state of activation without signals from infiltrating T cells. This study aims to isolate the immediate alterations in protective and inflammatory gene expression that occur in epidermal KCs, with a particular focus on molecules associated with cell-mediated immunity. We used dispase-separated epidermis, followed by intercellular disassociation by trypsinization, as a model for epidermal injury. We obtained a pure population of KCs using flow cytometry. As a control for

SUBMITTER: Kennedy-Crispin M 

PROVIDER: S-EPMC3235229 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature