Serum analysis of tryptophan catabolism pathway: correlation with Crohn's disease activity.

ABSTRACT:

Background

Indoleamine 2,3 dioxygenase-1 (IDO1) is a tryptophan catabolizing enzyme with immunotolerance-promoting functions. We sought to determine if increased gut expression of IDO1 in Crohn's disease (CD) would result in detectable changes in serum levels of tryptophan and the initial IDO1 pathway catabolite, kynurenine.

Methods

Individuals were prospectively enrolled through the Washington University Digestive Diseases Research Center. The Montreal Classification was used for disease phenotyping. Disease severity was categorized by the Physician's Global Assessment. Serum tryptophan and kynurenine were measured by high-pressure liquid chromatography. IDO1 immunohistochemical staining was performed on formalin-fixed tissue blocks.

Results

In all, 25 CD patients an

SUBMITTER: Gupta NK 

PROVIDER: S-EPMC3235239 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature