Glial Hsp70 protects K+ homeostasis in the Drosophila brain during repetitive anoxic depolarization.

ABSTRACT: Neural tissue is particularly vulnerable to metabolic stress and loss of ion homeostasis. Repetitive stress generally leads to more permanent dysfunction but the mechanisms underlying this progression are poorly understood. We investigated the effects of energetic compromise in Drosophila by targeting the Na(+)/K(+)-ATPase. Acute ouabain treatment of intact flies resulted in subsequent repetitive comas that led to death and were associated with transient loss of K(+) homeostasis in the brain. Heat shock pre-conditioned flies were resistant to ouabain treatment. To control the timing of repeated loss of ion homeostasis we subjected flies to repetitive anoxia while recording extracellular [K(+)] in the brain. We show that targeted expression of the chaperone protein Hsp70 in glial cells dela

SUBMITTER: Armstrong GA 

PROVIDER: S-EPMC3236231 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature