Identification of maturation and protein synthesis related proteins from porcine oocytes during in vitro maturation.

ABSTRACT:

Background

In vitro maturation (IVM) of mammalian oocytes is divided into the GV (germinal vesicle stage), MI (metaphase I stage) and MII (metaphase II stage) stages, and only fully mature oocytes have acquired the ability to be fertilized and initiate zygotic development. These observations have been mostly based on morphological evaluations, but the molecular events governing these processes are not fully understood.The aim of the present study was to better understand the processes involved in the molecular regulation of IVM using 2-DE analysis followed by mass spectrometry to identify proteins that are differentially expressed during oocyte IVM.

Result

A total of 16 up-regulated and 12 down-regulated proteins were identified. To investigate the IVM process, we specifical

SUBMITTER: Kim J 

PROVIDER: S-EPMC3236306 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature